Chromatin is often overlooked during viral vector downstream processing, yet it can have a significant impact on recovery, filtration performance, process robustness, and manufacturing economics. This scientific poster explores why nuclease selection plays a critical role in chromatin clearance and how it can influence overall process performance.
In this poster, you'll learn:
- Why residual host-cell DNA is primarily chromatin rather than free DNA.
- How salt conditions can limit the performance of conventional nucleases during downstream processing.
- The downstream consequences of incomplete chromatin digestion, including filter fouling, aggregation, and recovery variability.
- How salt-stable nucleases can improve chromatin clearance and process robustness.
- The potential impact of nuclease selection on recovery, processing efficiency, DNA clearance, and manufacturing costs.
Download the poster to explore the scientific and economic impact of chromatin clearance in viral vector manufacturing
