Name: ArcticZymes Proteinase
Brand: ArcticZymes Technologies

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ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.

Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.

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Key Features

Easy to inactivate after use: 15 - 30 min at 55 - 60°C
Broad substrate specificity
Active at high salt concentrations
Compatible with downstream DNA analyses
Available in Glycerol FREE formulation

Applications

Lysis and releasing nucleic acid from single-cells and small tissue samples
Optimization of DNA/RNA yields from nucleic acids when added to extraction procedures

Figures

Figure 1. AZ Proteinase tolerates GTC at high concentrations

Works well in single cell lysis buffers (*GTC: 50% at 400 mM)

*The use of Guanidine Thiocyanate (GTC) solution in direct cell lysis buffers is patented by TATAA Biocenter AB / Roche

Figure 2. Easy to inactivate after use

Remaining activity after incubation of ArcticZymes Proteinase for 30 minutes at different temperatures.

Figure 3. ArcticZymes Proteinase improves detection of gDNA in single cells preparations

The indicated number of human cells were lysed in 200 μl single cell lysis buffer supplemented with 7.5 U/ml AZ Proteinase using no-proteinase buffer as reference. 1 μl of lysate was used directly in 10 μl qPCR reactions to quantify human gDNA.

Properties

Source

Recombinantly produced in Pichia pastoris

Size

34 kDa

Unit Definition

One unit is defined as the amount of enzyme that produces one µmol 4-nitroaniline (extinction coefficient 8.8 mM-1 at 410 nm) at 25°C, pH 8.0 per minute (buffer conditions 50 mM Tris-HCl pH 8.0, 1% DMSO, 1 mM Suc-Ala-Ala-Pro-Phe-pNA).

Specificity

Nonspecific endopeptidase

Activity

Highly active in temperature range of 25–40°C
pH: 7–10

Tolerance to Typical Buffer Additives

SDS: 0.2–1%

Inhibitors

The enzyme is inhibited by general serine protease inhibitors, such as PMSF.

Storage

ArcticZymes Proteinase is stable at -20°C for up to 2 years in the supplied storage buffer. ArcticZymes Proteinase Glycerol FREE is stable at -20°C for up to 1 year in the supplied storage buffer.