ArcticZymes Proteinase
ArcticZymes Proteinase
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ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.
Histones and other proteins areknown to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.
Key Features
- Easy to inactivate after use: 15 - 30 min at 55 - 60°C
- Broad substrate specificity
- Active at high salt concentrations
- Compatible with downstream DNA analyses
- Available in Glycerol FREE formulation
Applications
- Lysis and releasing nucleic acid from single-cells and small tissue samples
- Optimization of DNA/RNA yields from nucleic acids when added to extraction procedures
Figures
Works well in single cell lyses buffers (*GTC: 50% at 400 mM)
*The use of Guanidine Thiocyanate (GTC) solution in direct cell lysis buffers is patented by TATAA Biocenter AB / Roche
Remaining activity after incubation of ArcticZymes Proteinase for 30 minutes at different temperatures.
The indicated number of human cells were lysed in 200 μl single cell lysis buffer supplemented with 7.5 U/ml AZ Proteinase using no-proteinase buffer as reference. A 1 μl of lysate was used directly in 10 μl qPCR reactions to quantify human gDNA.
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