Name: RiboClean™ dsDNase
Brand: ArcticZymes Technologies

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Early access program

Test RiboClean™ in your own workflow

Early product access
Technical support from ArcticZymes specialists
Protocol guidance and workflow recommendations
Opportunity to provide feedback ahead of the full ISO batch production scheduled for 2027*

Early access offer

15% off

during the early access phase

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*Early access products are supplied for research use only. Formulation, recommended reaction conditions and specifications may change before full ISO release, following customer feedback collected during the early-access phase. We will notify early-access customers of any changes.

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RiboClean™ dsDNase is a double-strand specific DNase designed for effective removal of template DNA from in vitro transcription (IVT) reactions. The enzyme selectively degrades double-stranded DNA while preserving RNA integrity and yield, enabling streamlined RNA manufacturing workflows with less DNase protein going into purification. Under tested IVT conditions, RiboClean™ dsDNase achieved template DNA digestion using up to 10× fewer enzyme units than conventional DNase I.

Key Benefits

Removes template DNA with up to 10x fewer enzyme units than DNase I.
Reduces residual template DNA while maintaining RNA integrity and yield.
Allows 40x less DNase protein by mass than DNase I, reducing DNase carry-over into purification.
Integrates directly into IVT workflows without additional calcium supplementation or buffer exchange.
Modeled >80% lower DNase-related cost per 1g batch, Modeled >10% lower reagent cost per mg RNA produced.

Key Features

Generates DNA fragments <17 nt.
Optimal activity between 35–45°C.
Active across a broad pH range and tolerant to variable process conditions.

Figures

Figure 1. RiboClean™ dsDNase has optimum activity between 0– 10 mM sodium chloride. It remains active up to 100 mM sodium chloride.

The activity was measured after 20 min of reaction at 37°C in perchloric acid assay with final pH 7.5, 5 mM MgCl₂, and with varying concentrations of NaCl.

Average of 3 experiments.

Figure 2. RiboClean™ dsDNase has an optimal activity when more than 4 mM magnesium chloride is present.

The activity was measured after 20 min of reaction at 37°C in perchloric acid assay with final pH 7.5 and varying MgCl₂ concentrations.

Average of 3 experiments.

Figure 3. RiboClean™ dsDNase has optimum activity between 35–45°C. It is active starting at 20°C.

The activity was measured after 20 min of reaction in perchloric acid assay with final theoretical pH 7.5 at measured temperatures and 5 mM MgCl₂.

Average of 2 experiments.

Figure 4. RiboClean™ dsDNase performs best in the 6.6–8.0 pH range. It is active starting at pH 6.1, up to pH 8.7.

The activity was measured after 20 min of reaction at 37°C in perchloric acid assay with 5 mM MgCl₂ and varying pH.

Average of 3 experiments.

Figure 5. RiboClean™ dsDNase has specific activity against double strand DNA.

The activity was measured over 10 min at 37 °C in reaction buffer with 25 mM Tris-HCl pH 7.5, 5 mM MgCl₂ and 0.1 µM of varying nucleic acid substrates and measured in a real-time on fluorescent assay. All values are blank corrected.

Average of 2 experiments.

Properties

Source

Recombinantly produced in Pichia pastoris

Specific Activity

ca. 80 000 units/mg

Protein Purity

> 90% by SDS-PAGE analysis

Unit Definition

One unit is defined as the amount of enzyme causing an increase in absorbance at 260 nm of 0.005 per minute at 25°C.

Specificity

Specific activity against double-stranded DNA.
Negligible activity toward ssDNA, ssRNA, and dsRNA.

Working Ranges

Temperature: 20 – 45°C, optimal: 35 – 45°C
Salt concentrations (NaCl): 0 – 100 mM, optimal: 0 mM – 10 mM
Mg²⁺: >0.5 mM is required for activity, optimal: ≥4 mM
pH: 6.1 – 8.7, optimal: 6.6 – 8.0

Note: The working range is defined as above 10% activity and optimal range as above 80% activity.

Recommended Protocols

Ordering Info

Quantity

Product Name

SKU

Pack Size

Concentration

Price

RiboClean™ dsDNase (15% discount included)

74200-202-PROT

500 U

5 U/µL

$ 347.00 USD

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RiboClean™ dsDNase (15% discount included)

74200-105-PROT

5 kU

5 U/µL

$ 2,084.00 USD

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Note: Article no. 71502-202 and 71502-120 are provided with IsoPol® BST⁺ 10X Buffer and MgSO_{4 .}‍

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Early access program

Test RiboClean™ in your own workflow

Early product access
Technical support from ArcticZymes specialists
Protocol guidance and workflow recommendations
Opportunity to provide feedback ahead of the full ISO batch production scheduled for 2027*

Early access offer

15% off

during the early access phase

Request early access

*Early access products are supplied for research use only. Formulation, recommended reaction conditions and specifications may change before full ISO release, following customer feedback collected during the early-access phase. We will notify early-access customers of any changes.