Isothermal High Temperature Range

Name: Isothermal High Temperature Range
Brand: ArcticZymes Technologies

IsoPol® BST⁺ For point-of-care diagnostic assays

Isothermal High Temperature Range

IsoPol® BST⁺ Isothermal High Temperature Range

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These DNA polymerases are ideal for Isothermal Amplification

IsoPol® BST⁺ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST⁺ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.

Key features

IsoPol® BST⁺ lacks 5’-3’- and 3’-5’-exonuclease activity.
Inactivation - IsoPol® BST⁺ is completely inactivated after one minute incubation at 95°C
Enhanced strand displacement and processivity.
Increased salt tolerance.

Key features

High concentration
High purity
Lyophilisation and automation friendly

Figures

Figure 1. IsoPol® BST⁺ shows enhanced strand displacement activity

IsoPol^®BST⁺ shows enhanced strand displacement activity compared to Bst polymerase. Activities at different concentrations of KCl at 37°C relative to activity at 50 mM KCl are shown for each polymerase.

Figure 2. IsoPol® BST⁺ tolerates higher concentrations of salt compared to Bst polymerase.

IsoPol® BST⁺ tolerates higher concentrations of salt compared to Bst polymerase. Polymerase activity of Bst and IsoPol® BST⁺ as function of NaCl data represented relative to highest activity of each polymerase.

Figure 3. IsoPol® BST⁺ has superior strand displacement activity

IsoPol® BST⁺ has superior strand displacement activity compared to Bst LF. SDA was measured at 37°C using a nicked dsDNA template.

Figure 4. IsoPol® BST⁺ has enhanced processivity compared to Bst LF

IsoPol® BST⁺ has enhanced processivity compared to Bst LF. Processivity was determined at 37°C using a primed circular ssDNA.

Figure 5. IsoPol® BST⁺ provides faster amplification in LAMP

Figure 6. IsoPol® BST⁺ provides superior time-to-results in RT-LAMP

IsoPol® BST⁺ results in faster detection of target in both LAMP and RT-LAMP. When IsoPol® BST⁺ was used in LAMP and RT-LAMP, time to results were strongly reduced compared to the non-engineered backbone, Bst LF. IsoPol® BST⁺ also significantly outperformed engineered versions of Bst LF from another vendor (Bst Vendor A). LAMP was used to detect 0.05 ng of λ DNA (Fig 5). RT-LAMP was performed with AMV RT to detect 10 ng of MS2 RNA template (Fig 6). LAMP and RT-LAMP were performed at 65°C.

Figure 7. IsoPol® BST⁺ tolerates serum

IsoPol® BST⁺ polymerase has high tolerance to matrix effects. LAMP was performed using λ DNA template in presence of human serum demonstrating that IsoPol® BST⁺ maintains high activity over a wide range of serum concentrations. No amplification was detected in the absence of template.