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Essential insights based on 3rd party publications:

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Quantification Challenges of Chromatin — Associated Host Cell DNA:
An Underestimated Risk

  • Discover why host cell DNA quantities are often significantly underestimated.
  • Understand the limitation of conventional nucleases when degrading chromatin

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interactive Q&A

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The Largest Overlooked Cost Driver in Viral Vector Production

  • Up to half of produced vector is lost during Down Stream Processing(DSP)
  • The majority of manufacturing cost accumulates is DSP raw materials
  • Nuclease design decisions determine downstream economics

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Advanced Therapies

Enzymes for RNA manufacturing and analytics

Reduce time and cost in post-⁠IVT template DNA removal and 5′ cap-⁠efficiency analysis.

The RNA workflow, step by step
DNA Template
IVT
(In Vitro Transcription)
Template DNA Digestion
RiboClean™
dsDNase
Purification
RNA QC & Characterization
RiboCleave™ 1
ssRNA restriction enzyme
Process Decision
Step 1 of 6
Process step

DNA Template

Every microgram of template DNA that goes into IVT has to be removed downstream.
The challenge

Template DNA Digestion: addressed with RiboClean™

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Step 2 of 6
Process step

IVT

IVT produces the RNA, but the template DNA remains and must be removed before downstream processing.

Next bottleneck in your workflow

Template DNA Digestion: addressed with RiboClean™

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Step 3 of 6
Common bottleneck

Template DNA Digestion

The problem
Residual template DNA has to be removed after IVT, and conventional DNase I often needs high enzyme input to get there.
Drives the challenge of: RNA quality, cost per batch
The impact
- Regulatory and RNA quality risk
- More nuclease carried into purification
- Higher cost per batch

10x

less enzyme units than DNase I

>80%

lower modeled DNase cost per batch
The solution at this step
dsDNA-specific nuclease for post-IVT cleanup
~6 hr → <30 min
Sample preparation
  • Single-enzyme, one-incubation workflow
  • Compatible with tested therapeutically relevant modified uridines
  • Supports Urea-PAGE, Fragment Analysis and LC-MS workflows
View RiboClean™
Request early access
Step 4 of 6
Process step

Purification

RNA is purified from enzymes, nucleotides and DNA fragments. Lower nuclease input means less DNase protein entering this step.
Next bottleneck in your workflow

RNA QC & Characterization: addressed with RiboCleave™ 1

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Step 5 of 6
Common bottleneck

RNA QC & Characterization

The problem
RNase H cap-analysis sample prep needs guide-oligo design, hybridization and multiple steps, and the readout typically relies on LC-MS.
Creates avoidable analytical time and complexity
The impact
- Approximately 6 hours of sample preparation
- Construct-specific guide-oligo workflow
- Slower analytical decisions
- Reliance on specialist instrumentation

~6 hr → <30 min

cap-efficiency sample preparation

>80%

lower modeled analytical cost vs RNase H
The solution at this step
Sequence-specific ssRNA restriction enzyme for cap-efficiency sample preparation
~6 hr → <30 min
Sample preparation
  • Single-enzyme, one-incubation workflow
  • Compatible with tested therapeutically relevant modified uridines
  • One digest, read by urea-PAGE, Bioanalyzer or LC-MS
View RiboClean™
Request early access
Step 6 of 6
Process step

Process Decision

QC results inform the next decision: release, further optimization or scale-up. Faster, simpler analytics can support faster decisions.
Workflow complete

Ready to test the interventions?

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The challenge

What’s holding RNA manufacturing back

RNA teams repeatedly encounter three sources of burden across manufacturing and analysis. Two high-value workflow steps contribute disproportionately to them.

RNA quality & regulatory readiness

Residual template DNA, process impurities and inconsistent analytics can complicate RNA quality and regulatory readiness.

Slow development & decisions

Lengthy digestion and analytical preparation can slow optimization, development and decision-making.

Manufacturing & analytical cost

High enzyme input and complex analytical preparation can increase cost per batch and per assay.
The solutions

Two enzymes. Two high-⁠value workflow interventions.

Each enzyme is designed around a specific source of avoidable burden, so we can improve the workflow without reinventing it.

Step 3 · Template DNA Digestion
Post-IVT template DNA removal
Up to 10× Fewer Units
Under the tested IVT comparison
  • Added directly to IVT, no buffer exchange or calcium
  • No detectable impact on RNA integrity or yield
  • 40x less DNase protein by mass into purification
View product
See the data · Download poster
Step 5 · RNA QC & Characterization
Cap-efficiency sample preparation
~6 hr → <30 min
Sample preparation
  • No guide oligo
  • One-enzyme digestion
  • Compatible with urea-PAGE, Bioanalyzer and LC-MS workflows
View product
See the data · Download poster
15%
Early Access Program: 15% discount
A 15% discount for teams evaluating RiboClean™ or RiboCleave™ 1 in their own workflow.
Contact us about the early access program
Proof in the workflow

See the data

PSTR
RiboClean™ dsDNase

Post-IVT template DNA removal with up to 10× fewer enzyme units

See how RiboClean™ dsDNase removes post-IVT template DNA with up to 10× fewer enzyme units while maintaining RNA integrity and yield.
Download poster
PSTR
RiboCleave™ 1

mRNA cap-efficiency sample preparation: ~6 hours to <30 minutes

One sequence-specific digestion replaces the multistep RNase H sample-preparation workflow, with the same digest read by urea-PAGE, Bioanalyzer or LC-MS.
Download poster
Proof in the workflow

All Advanced Therapies resources

Posters, white papers, application notes and webinars in the ArcticZymes Resource Center.
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Early access program

Test them in your own workflow

Work with our RNA enzyme specialists to evaluate RiboClean™ or RiboCleave™ 1 against your current process or analytical workflow.

  • Early product access
  • Technical support from ArcticZymes specialists
  • Protocol guidance and workflow recommendations
  • Opportunity to provide feedback ahead of full ISO batch production*
15% early access pricing

Which step is worth testing first?

Next Step
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An ArcticZymes RNA enzyme specialist will contact you about your workflow. See our privacy policy.
*Early access products are supplied for research use only. Formulation, recommended reaction conditions and specifications may change before full ISO release, following customer feedback collected during the early access phase. We will notify early-access customers of any changes.

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