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AZtaq™ DNA Polymerase

AZtaq™ DNA Polymerase

AZtaq™ DNA Polymerase

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AZtaq™ DNA Polymerase is a high-quality DNA polymerase, originating form Thermus aquaticus. Being highly thermostable, AZtaq is ideal for use in polymerase chain reaction (PCR) applications.

The enzyme catalyses the synthesis of a complementary DNA strand using a primed DNA or cDNA strand as template. It possesses 5’-3’ exonuclease activity while lacking 3’-5’ proofreading activity.

AZtaq is compatible with the use of dUTP, enabling highly efficient removal of carry-over contamination with Cod UNG.

Key Features 

  • Excellent qPCR Performance
  • Compatible with dUTP
  • Thermostable

Applications

  • PCR/qPCR

Figures

AZtaq DNA Polymerase - Figure 2
Figure 1.2. AZtaq shows excellent performance and sensitivity in qPCR
AZtaq DNA Polymerase - Figure 2

A serial dilution of M13KO7 DNA was used as template for qPCR amplification using specific primers together with a fluorescently labelled hydrolysis probe detecting the M13v1.1 target. Data showing amplification plots (A) and standard curve (B) for the qPCR reactions containing from 1 000 000 down to 1 copy of M13v1.1 target DNA, in triplicate measurements.

Properties

Specifications
Source

Recombinantly produced in E. coli

Size

95.1 kDa

Unit Definition

One unit (U) is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 min at 72°C. The enzyme is assayed in 20 mM Tris-HCl pH 8.8, 55 mM KCl, 2 mM MgCl2, 0.02 mg/ml BSA, 15 nM primed M13mp18 ssDNA, 0.8 mM dNTPs (0.2 mM each), 1.888 µM 3H-dTTP.

Inactivation

N/A

Storage and Stability

AZtaq is stable at -20°C for up to 1 year in the supplied storage buffer. Additional data on stability is available on request.

Quality Control

ArcticZymes is dedicated to the quality of our products. AZtaq is manufactured at our ISO 13485 certified facility in Norway.

dsDNA endonuclease activity

20 U AZtaq was incubated with a supercoiled plasmid (1 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any transformation of closed circular DNA to nicked DNA.

ssDNA endonuclease activity

20 U AZtaq was incubated with M13 ssDNA (0.5 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any visible signs of ssDNA degradation.

dsDNA exonuclease activity

20 U AZtaq was incubated with 3H-dATP labelled dsDNA (0.5 µg, 500 bp) for 4 hrs at 37°C. Acid soluble radioactivity from labelled DNA was not significantly over blank test for either substrate.

RNase activity

20 U AZtaq was incubated with a 2 kb RNA transcript (1 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any visible signs of RNA degradation.

E. coli gDNA

5 U AZtaq was analysed in a probe-based qPCR assay detecting the 23S ribosomal RNA gene in E. coli. Less than 3 copies of E. coli gDNA were detected.

Functional test

5 Units AZtaq was used in a qPCR assay amplifying a serial dilution of down to < 3 copies of E. coli gDNA using specific primers detecting the 23S ribosomal RNA gene. Successful amplification was observed for all dilutions. The resulting standard curve showed at least 90-110 % efficiency, indicating efficient doubling of specific template product per cycle.

Recommended Protocols

Ordering Info

Quantity
Product Name
SKU
Pack Size
Concentration
Price
1
T4 DNA Ligase
71800-202
250 kU
5000 U/μl
€ 0.00 EUR
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1
AZscript RT
72100-201
10000 U
200 U/μl
€ 119.00 EUR
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1
Heat&Run gDNA Removal Kit
50200-250
250 rxn
€ 531.00 EUR
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1
M-SAN HQ ELISA Kit
70950-001
12x8 Strip plate
€ 635.00 EUR
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1
HL-dsDNase Triton Free
70801-201
250 U
2U/µl
€ 296.00 EUR
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1
PCR Decontamination Kit
80400-100
100 rxn
€ 107.00 EUR
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1
AZtaq™ DNA Polymerase
73100-201
500 U
5 U/µl
€ 73.00 EUR
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1
ArcticZymes Proteinase
71600-201
50 U
>200 U/ml
€ 127.00 EUR
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1
IsoPol®
71500-201
200 U
5U/µl
€ 66.00 EUR
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1
HL-SAN Triton Free
70911-150
500 kU
25
€ 0.00 EUR
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1
ArcticZymes R2D Ligase
71900-105
5 kU
10 U/µl
€ 350.00 EUR
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1
rSAP
70700-201
1000 U
1 U/µl
€ 112.00 EUR
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1
dsDNase Triton Free
70601-201
250 U
2 U/µl
€ 296.00 EUR
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1
Cod UNG Triton Free
70501-201
100 U
1 U/µl
€ 106.00 EUR
Request Quote
1
IsoPol™ BST⁺
71502-202
1600 U
8 U/µl
€ 69.00 EUR
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1
SAN High Quality
70920-150
500 kU
>250 U/µl
€ 0.00 EUR
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1
HL-ExoI
70100-201
2500 U
>20 U/µl
€ 63.00 EUR
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1
SAN HQ 2.0
70940-150
500 kU
250 U/µl
€ 0.00 EUR
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2
Cod UNG Triton Free
70501-202
1000 U
1 U/µl
€ 442.00 EUR
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2
SAN High Quality
70920-160
5 MU
>250 U/µl
€ 0.00 EUR
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2
PCR Decontamination Kit
80400-500
500 rxn
€ 431.00 EUR
Request Quote
2
HL-dsDNase Triton Free
70801-202
1000 U
2U/µl
€ 850.00 EUR
Request Quote
2
IsoPol™ BST⁺
71502-120
10000 U
120 U/µl
€ 339.00 EUR
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2
dsDNase Triton Free
70601-202
1000 U
2 U/µl
€ 850.00 EUR
Request Quote
2
HL-SAN Triton Free
70911-202
25000 U
25 U/µl
€ 458.00 EUR
Request Quote
2
T4 DNA Ligase
71800-100
as per request
as per request
€ 0.00 EUR
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2
ArcticZymes R2D Ligase
71900-100
As per request
As per request
€ 0.00 EUR
Request Quote
2
M-SAN HQ
70950-120
200 kU
>250 U/µl
€ 0.00 EUR
Request Quote
2
IsoPol® SD+
71501-201
200 U
5U/µl
€ 66.00 EUR
Request Quote
2
rSAP
70700-202
5000 U
1 U/µl
€ 436.00 EUR
Request Quote
2
SAN HQ 2.0
70940-160
5 MU
>250 U/µl
€ 0.00 EUR
Request Quote
2
Heat&Run gDNA Removal Kit
80200-50
50 rxn
€ 135.00 EUR
Request Quote
2
HL-ExoI
70100-202
500 kU
>20 U/µl
€ 0.00 EUR
Request Quote
2
AZscript RT
72100-100
As per request
As per request
€ 0.00 EUR
Request Quote
2
ArcticZymes Proteinase
71600-110
1000 U
>200 U/ml
€ 1,151.00 EUR
Request Quote
2
AZtaq™ DNA Polymerase
73100-110
5000 U
>50 U/µl
€ 550.00 EUR
Request Quote
3
Cod UNG Triton Free
70501-110
10 kU
1 U/µl
€ 0.00 EUR
Request Quote
3
SAN HQ 2.0
70940-202
25 kU
25 - 30 U/µl
€ 483.00 EUR
Request Quote
3
rSAP
70700-203
25 kU
1 U/µl
€ 0.00 EUR
Request Quote
3
HL-ExoI
70100-150
50 kU
>20 U/µl
€ 0.00 EUR
Request Quote
3
M-SAN HQ
70950-150
500 kU
>250 U/µl
€ 0.00 EUR
Request Quote
3
ArcticZymes Proteinase
71600-100
As per request
>200 U/ml
€ 0.00 EUR
Request Quote
3
IsoPol® BST⁺ Glycerol Free
71512-100
As per request
As per request
€ 0.00 EUR
Request Quote
3
HL-dsDNase Triton Free
70801-203
2500 U
5U/µ
€ 1,900.00 EUR
Request Quote
3
AZtaq™ DNA Polymerase
73100-100
As per request
As per request
€ 0.00 EUR
Request Quote
3
dsDNase Triton Free
70601-203
2500 U
5U/µl
€ 1,900.00 EUR
Request Quote
3
SAN High Quality
70920-202
25 kU
25-30 U/µl
€ 483.00 EUR
Request Quote
4
IsoPol® BST⁺ Glycerol HC Free
71522-100
As per request
As per request
€ 0.00 EUR
Request Quote
4
Cod UNG Triton Free
70501-151
50 kU
1 U/µl
€ 0.00 EUR
Request Quote
4
ArcticZymes Proteinase Glycerol-FREE
71610-100
As per request
>200 U/ml
€ 0.00 EUR
Request Quote
4
rSAP
70700-101
200 kU
1 U/µl
€ 0.00 EUR
Request Quote
4
SAN High Quality Triton Free
70921-150
500 kU
>250 U/µl
€ 0.00 EUR
Request Quote
4
dsDNase Triton Free
70601-150
50 kU
50U/µl
€ 0.00 EUR
Request Quote
4
SAN HQ 2.0 ELISA Kit
70970-001
1x96 Well Plate
€ 635.00 EUR
Request Quote
4
HL-dsDNase Triton Free
70801-110
100 kU
2 U/µl
€ 0.00 EUR
Request Quote
5
Cod UNG Triton Free
70501-150
50 kU
50 U/µl
€ 0.00 EUR
Request Quote
5
HL-dsDNase Glycerol-Free - Triton Free
70811-50
5000 U
>50 U/µl
€ 0.00 EUR
Request Quote
5
rSAP Glycerol-free
70710-201
5000 U
>20 U/µl
€ 505.00 EUR
Request Quote
4
M-SAN HQ
70950-155
1 MU
>250 U/µl
€ 0.00 EUR
Request Quote
5
SAN High Quality Triton Free
70921-160
5 MU
>250 U/µl
€ 0.00 EUR
Request Quote
5
M-SAN HQ
70950-160
5 MU
>250 U/µl
€ 0.00 EUR
Request Quote
5
dsDNase Triton Free
70601-110
100 kU
2U/µl
€ 0.00 EUR
Request Quote
6
rSAP Glycerol-free
70710-100
As per request
As per request
€ 0.00 EUR
Request Quote
6
Cod UNG Glycerol-Free Triton Free
70511-105
5000 U
50 U/µl
€ 0.00 EUR
Request Quote
6
SAN High Quality Triton Free
70921-202
25 kU
25-30 U/µl
€ 483.00 EUR
Request Quote
6
dsDNase Glycerol Free, Triton Free
70611-50
5000 U
>50 U/µl
€ 0.00 EUR
Request Quote
6
M-SAN HQ
70950-202
25 kU
25-30 U/µl
€ 562.00 EUR
Request Quote
6
HL-dsDNase
70800-201
250 U
2 U/µl
€ 296.00 EUR
Request Quote
7
Cod UNG Glycerol-Free Triton Free
70511-150
50 kU
50 U/µl
€ 0.00 EUR
Request Quote
7
SAN High Quality ELISA Kit
70930-001
1x96 Well Compact Plate Format
€ 635.00 EUR
Request Quote
7
dsDNase
70600-201
250
2 U/µl
€ 296.00 EUR
Request Quote
7
HL-dsDNase
70800-202
1000 U
2 U/µl
€ 850.00 EUR
Request Quote
8
Cod UNG
70500-201
100 U
1 U/µl
€ 106.00 EUR
Request Quote
8
dsDNase
70600-202
1 000
2 U/µl
€ 850.00 EUR
Request Quote
8
HL-dsDNase
70800-203
2500 U
5 U/µl
€ 1,900.00 EUR
Request Quote
9
Cod UNG
70500-202
1000 U
1 U/µl
€ 442.00 EUR
Request Quote
9
dsDNase
70600-203
2 500
€ 1,900.00 EUR
Request Quote
9
HL-dsDNase
70800-150
50 kU
50 U/µl
€ 0.00 EUR
Request Quote
10
dsDNase
70600-150
50 kU
50U/µl
€ 0.00 EUR
Request Quote
10
Cod UNG
70500-110
10 kU
1 U/µl
€ 0.00 EUR
Request Quote
10
HL-dsDNase
70800-110
100 kU
2 U/µl
€ 0.00 EUR
Request Quote
11
dsDNase
70600-110
100 kU
2U/µl
€ 0.00 EUR
Request Quote
11
Cod UNG
70500-151
50 kU
1 U/µl
€ 0.00 EUR
Request Quote
11
HL-dsDNase
70800-120
500 kU
2 U/µl
€ 0.00 EUR
Request Quote
12
Cod UNG
70500-150
50 kU
50 U/µl
€ 0.00 EUR
Request Quote
12
dsDNase Glycerol Free
70610-50
5000 U
80 - 140 U/µl
€ 0.00 EUR
Request Quote
12
HL-dsDNase Glycerol-free
70810-50
5000 U
80 - 140 U/µl
€ 0.00 EUR
Request Quote
13
Cod UNG Glycerol Free
70510-105
5000 U
50 U/µl
€ 0.00 EUR
Request Quote
14
Cod UNG Glycerol Free
70510-150
50 kU
50 U/µl
€ 0.00 EUR
Request Quote
Note: Article no. 71502-202 and 71502-120 are provided with IsoPol® BST+ 10X Buffer and MgSO4 .
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AZtaq™ DNA Polymerase