M-SAN HQ (for Diagnostics)
No Other Endonuclease Performs Better at Physiological Conditions
M-SAN HQ (for Diagnostics)
OverView
Applications
M-SAN HQ - Optimum Depletion of Host Cells DNA
M-SAN HQ is selected for workflows that demand reliable and effiecient host DNA depletion without compromising sensitive pathogen detection, including RNA viruses. It works directly in minimally processed samples such as plasma, swabs, or urine at physiological salt concentrations (125 to 250 mM NaCl).
In some cases, high concentrations of salt can reduce recovery of fragile pathogens like enveloped RNA viruses. M SAN HQ is exceptionally active at physiological salt levels, making it the obvious choice when the goal is to detect and analyse both bacterial and viral DNA/RNA from the same sample. This enables unified RNA and DNA detection from a single sample. No prior purification is needed.
When supplemented with low amounts of Magnesium, M-SAN HQ works efficiently directly in most patient samples (physiological salt and pH) without the need for buffer adjustments.
Key Benefits
- Broad workflow compatibility: Supports unified RNA and DNA metagenomics or DNA-only diagnostics across viral and bacterial pathogen targets
- Optimum activity: Active at physiological salt and pH levels for direct use in sample
- Quality: Maintains the integrity of labile biological material, supporting accurate downstream analysis
- Flexibility: Compatible with a wide range of sample types and media, simplifing workflow design
- Tired and trusted: Validated and proven effective in real-world diagnotic metagenomic wo
Quality Supporting Diagnostics
ArcticZymes is dedicated to the quality of our products. M-SAN HQ is manufactured following the ISO 13485 Quality Management System.
Figures
M-SAN High Quality has optimum activity between 125 - 250 mM NaCl, which correlates to the salt content in buffers with physiological salinity. The activity was measured at 37°C in a 25 mM Tris-HCl buffer, pH 7.5, 5 mM MgCl2 and varying concentrations of NaCl. Maximum activity was set to 100%.
M-SAN HQ has optimal performance in the 7.2 - 8.7 pH range. This allows very high performance in most biological buffers. With a working range starting at pH 6.5, M-SAN HQ is also a candidate for DNA removal from insect cell media. The activity was measured at 37°C in a 25 mM Tris-HCl or Bis-Tris buffer at various pH, 2.5 mM MgCl2 and 150 mM NaCl. Activity measured at pH 7.7 was set to 100%.
M-SAN HQ is highly efficient for digesting DNA at 37°C, and tolerate temperatures up to 50°C. The activity was measured in a 25 mM Tris-HCl buffer at pH 7.8 (@measurement temperature), 5 mM MgCl2 and 150 mM of NaCl. 37°C was set to 100% as this is the temperature used for defining the activity in Units.
Properties
Recommended Protocols
Ordering Info
Considering M-SAN HQ in your clinical metagemonics kit? Contact us for larger volume pack sizes and pricing details.
Powered by Bioz
