M-SAN HQ (for Diagnostics)

No Other Endonuclease Performs Better at Physiological Conditions

M-SAN HQ (for Diagnostics)

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Applications ‍

‍Host Cell DNA Depletion Prior to Diagnostic Metagenomic Analysis

M-SAN HQ - Optimum Depletion of Host Cells DNA

M-SAN HQ is selected for workflows that demand reliable and effiecient host DNA depletion without compromising sensitive pathogen detection, including RNA viruses. It works directly in minimally processed samples such as plasma, swabs, or urine at physiological salt concentrations (125 to 250 mM NaCl).

In some cases, high concentrations of salt can reduce recovery of fragile pathogens like enveloped RNA viruses. M SAN HQ is exceptionally active at physiological salt levels, making it the obvious choice when the goal is to detect and analyse both bacterial and viral DNA/RNA from the same sample. This enables unified RNA and DNA detection from a single sample. No prior purification is needed.

When supplemented with low amounts of Magnesium, M-SAN HQ works efficiently directly in most patient samples (physiological salt and pH) without the need for buffer adjustments.

Key Benefits

Broad workflow compatibility: Supports unified RNA and DNA metagenomics or DNA-only diagnostics across viral and bacterial pathogen targets
Optimum activity: Active at physiological salt and pH levels for direct use in sample
Quality: Maintains the integrity of labile biological material, supporting accurate downstream analysis
Flexibility: Compatible with a wide range of sample types and media, simplifing workflow design
Tired and trusted: Validated and proven effective in real-world diagnotic metagenomic wo

Quality Supporting Diagnostics

ArcticZymes is dedicated to the quality of our products. M-SAN HQ is manufactured following the ISO 13485 Quality Management System.

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Figures

Figure 1. Optimum activity in solutions at physiological salinity

M-SAN High Quality has optimum activity between 125 - 250 mM NaCl, which correlates to the salt content in buffers with physiological salinity. The activity was measured at 37°C in a 25 mM Tris-HCl buffer, pH 7.5, 5 mM MgCl₂ and varying concentrations of NaCl. Maximum activity was set to 100%.

Figure 2. Highly compatible with physiological pH

M-SAN HQ has optimal performance in the 7.2 - 8.7 pH range. This allows very high performance in most biological buffers. With a working range starting at pH 6.5, M-SAN HQ is also a candidate for DNA removal from insect cell media. The activity was measured at 37°C in a 25 mM Tris-HCl or Bis-Tris buffer at various pH, 2.5 mM MgCl₂ and 150 mM NaCl. Activity measured at pH 7.7 was set to 100%.

Figure 3. Superior performance at 37°C and above

M-SAN HQ is highly efficient for digesting DNA at 37°C, and tolerate temperatures up to 50°C. The activity was measured in a 25 mM Tris-HCl buffer at pH 7.8 (@measurement temperature), 5 mM MgCl₂ and 150 mM of NaCl. 37°C was set to 100% as this is the temperature used for defining the activity in Units.

Properties

Source

Recombinantly produced in Pichia pastoris

Molecular Weight

24.5 kDa

Protein Purity

> 99% by SDS-PAGE analysis

Isoelectric Point

8.62

Unit Definition

One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37° in 25 mM Tris-HCl pH 7.6 (@25°C), 2.5 mM MgCl₂, 150 mM NaCl, and 50 μg/ml calf thymus DNA.

Specificity

Nonspecific endonuclease cleaving single and double stranded DNA and RNA.

Working Ranges

Temperature: 20 – 50°C, optimal: 36 – 50°C
Salt concentrations (NaCI): 0 – 300 mM, optimal: 100 mM – 250 mM
Mg²⁺: > 0.5 mM is required for activity, optimal: 4 – 15 mM
pH: 6.5 – 9.5, optimal: 7.2 – 8.7

Note: The working range is defined as above 10% activity and optimal range as above 80% activity.

Tolerance to Common Buffer Additives

Tween 20: No reduction in activity (tested up to 10%)
Virodex TXR-1: No reduction in activity (tested up to 10%)
Virodex TXR-2: No reduction in activity (tested up to 10%)
Deviron 13-S9: No reduction in activity (tested up to 10%)
Deviron C16: No reduction in activity (tested up to 1%)
CHAPS: More than 50% activity up to 1% CHAPS (at 150 mM NaCl)
NaDeoxycholate: Not recommended
Pluronic F68/P188: More than 50 % activity up to 15 %
LDAO: No reduction in activity (tested up to 0.1%)
Trisodium citrate: 40% activity at 10 mM
Na₂HPO₄ (pH 7.5): No reduction in activity at 10 mM, 40% activity at 50 mM

Recommended Protocols

Ordering Info

Quantity

Product Name

SKU

Pack Size

Concentration

Price

T7 RNA Polymerase

74100-201

10 000 U

50 U/µl

$ 155.00 USD

M-SAN HQ (for Diagnostics)