Name: Shrimp Alkaline Phosphatase
Brand: ArcticZymes Technologies

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ArcticZymes' Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market.

Originally isolated from Pandalus borealis (Arctic shrimp), it has been purified from a recombinant source since 2010. Unlike other alkaline phosphatases, rSAP can be fully inactivated by a short heat treatment of 15 minutes at 65°C.

This added convenience through complete heat inactivation has made SAP one of the most sold DNA modifying enzymes.

For added flexibility, especially when lyophilisation may be desired, rSAP is also available in a Glycerol FREE format. First launched in 1993, SAP's unique features continue to make it a valuable tool in various applications.

Key Features

Gold standard heat labile, all-purpose alkaline phosphatase.
Completely inactivated after 5 min at 65°C or 1 min at 75°C.
Robust: Active in most restriction enzyme buffers, no need for supplemental zinc or other additives for activity.
Simple, hassle-free dephosphorylation of DNA, RNA, dNTPs and proteins for subsequent use in cloning or end-labelling of probes.
No vector purification necessary.
Easy inactivation of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis.
Excellent stability at 4°C and RT.

Applications

Dephosphorylation of vectors prior to cloning.
Enzymatic clean-up of PCR products before sequencing.
Ideal for MALDI-TOF, cloning, HLA typing, genotyping and sequencing.
Preparing substrate in protein kinase studies.

Figures

Figure 1. SAP Heat activation

Figure 2. SAP stability test

Properties

Unit Definition

One Unit will convert 1 µmol of p-nitrophenyl phosphate per minute to nitrophenol and phosphate at 37ºC and pH 10.4 in 0.1 M glycine buffer, 1 mM each of ZnCl₂ and MgCl₂ and 6 mM 4-nitrophenyl phosphate.

This Unit definition makes 1 Unit of SAP equivalent to 5 to 40 Units of Antarctic Phosphatase (New England Biolabs), 1.3 Units APex phosphatase or 35 Units NTPhos phosphatase (Epicentre Biotechnologies). SAP is therefore the most processive alternative.

Purity

DNase activities not detected.

Stability

Stable at -20ºC in storage buffer (25 mM Tris-HCl pH 7.6, 5 mM MgCl₂, glycerol 50%). At room temperature, >95% activity remains after 90 days. Still, the enzyme is completely inactivated after 5 minutes at 65°C. At 75°C, SAP is completely inactivated after only 1 minute. In a standard thermocycler, the process of heating from 37 to 95 and back to 37°C is sufficient to completely inactivate SAP.

Intellectual Property

Recombinant SAP is covered by the following patents: US 7,323,325, EP 1,326,890, JP 4,191,479, AU 9,398,701 and related applications in other countries either granted or pending.