Developed by researchers at the Austrian Centre of Industrial Biotechnology (ACIB), this poster examines how salt-active nuclease treatment can improve measles virus purification. The study compares conventional nuclease treatment with M-SAN HQ and shows how improved chromatin and DNA removal can increase both product purity and virus recovery.
Key Findings
- Why chromatin can be a major obstacle during purification of enveloped viruses.
- How salt-active nuclease treatment improved chromatin degradation and residual DNA removal.
- The effect of nuclease treatment on virus recovery, purity and infectivity.
- How heparin-affinity chromatography was used to purify measles virus from clarified cell culture supernatant.
- How ACIB researchers achieved improvements in both purity and yield using a simple, scalable purification workflow.
Access the full ACIB research poster to explore the data behind the study, including chromatography profiles, residual DNA analysis, infectivity measurements and purification results demonstrating the benefits of combining M-SAN treatment with heparin-affinity chromatography.
