False Confidence, Real Cost — How Chromatin Escapes Standard DNA Analytics and Undermines Your Downstream Process

New data reveals a problem in viral vector manufacturing that many bioprocessing teams do not know they have.

Your DNA assay returns a clean number. Your process looks compliant. So why are yields inconsistent, filters fouling ahead of schedule, and purification steps underperforming in ways that are hard to explain?

The answer may lie not in your downstream process — but in what your analytics never captured in the first place.

In viral vector manufacturing, residual host-cell DNA rarely exists as naked, freely accessible DNA. It exists as chromatin — histone-bound, structurally complex, and largely invisible to standard quantification methods like PicoGreen. When chromatin goes undetected at the measurement step, every process decision built on that data inherits the same blind spot.

New data from ACIB, publishing Spring 2026, shows the consequences showing up downstream — often disguised as something else entirely.

This webinar brings together scientists from ACIB and ArcticZymes to show you what the data reveals — and what to do about it.

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‍What you will learn

• Why standard DNA quantification methods systematically underestimate chromatin-associated DNA — and by how much.
• How chromatin's protein-bound structure defeats intercalating dyes and creates a false sense of analytical compliance.
• What sample preparation steps silently skew your DNA measurements before you even run the assay.
• How undetected chromatin co-purifies with your viral vectors through clarification, TFF, and chromatography.
• What the downstream consequences look like — and why they are so difficult to trace back to the analytics step.
• How to detect chromatin properly, and which nuclease strategies actually remove it under your process conditions.

Who should attend

This webinar is designed for scientists and technical leaders working in viral vector manufacturing who rely on DNA analytics to make process decisions — including process development scientists, downstream and purification scientists, CMC teams, and manufacturing leads at CDMOs and gene therapy companies.

This is the session your DNA clearance data has been waiting for.

If you have ever seen unexplained variability in your DSP performance, questioned whether your DNA clearance data truly reflects what is in your sample, or struggled to achieve consistent yields across batches, this session is directly relevant to your work.

About the collaboration

This webinar is based on peer-reviewed research conducted at ACIB, one of Europe's leading industrial biotechnology research institutes, in collaboration with ArcticZymes Technologies, the inventor of salt-active nucleases for biomanufacturing. Together, they have published independent, reproducible data showing how chromatin behaves in viral vector harvests, how standard nucleases fail to remove it, and what optimized nuclease strategies deliver in practice.

‍About acib:

The Austrian Centre of Industrial Biotechnology (acib) is a non-profit international research centre in the field of industrial biotechnology. The centre develops sustainable, and economically and technologically advanced processes for the biotech-, pharmaceutical and chemical industries.

About ArcticZymes:

ArcticZymes is the inventor Salt Active Nucleases (2015) and is the only provider of nucleases that perform optimally at both physiological and high-salt conditions. Our unique Biomanufacturing portfolio focuses on solutions that empower you to choose the nuclease that performs best, based on your conditions and your process.  Learn more about ArcticZymes: https://www.arcticzymes.com/about-us