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We Solved the Paradox!
High Salt Conditions Now Boost,
Not Hinder, DNA Clearing

The Salt & Nuclease Paradox: As salt concentration increases, conditions for DNA clearance improve, but enzyme function decreases. This “paradox” is resolved by SAN HQ GMP because its ability to clear DNA from viral vectors increases as salt concentration rises.

SAN HQ GMP performs best when conditions are optimal for clearing DNA. The results are maximum yield, purity, overall product quality, simplified workflow, reduced costs, and improved efficiency of DNA removal from your lysates in a single step.

Download the New Salt Active Nuclease Brochure

The graph explains the Salt and Nuclease Paradox
At low, or no, salt concentrations, chromatin sticks to vectors causing aggregates that prevent enzymes from clearing DNA efficiently. At high salt concentrations, aggregates dissolve, so enzymes can clear DNA efficiently, minimizing residual DNA and maximizing yield. But high salt reduces the function of Benzonase and Denarase from ca. 50% at 150mM salt to near 0% at salt levels where DNA is most accessible for clearing.

Advantages of increased salt levels using SAN HQ GMP
Optimized Residual DNA Removal

In high salt conditions, DNA can detach from viral capsids. However, conventional nucleases that digest this residual DNA struggle in such environments. The salt-tolerant SAN HQ GMP nuclease, though, maintains its functionality, paving the way for more effective DNA removal and superior alignment with regulatory standards.

In high salt conditions, DNA can detach from viral capsids. However, conventional nucleases that digest this residual DNA struggle in such environments. The salt-tolerant SAN HQ GMP nuclease, though, maintains its functionality, paving the way for more effective DNA removal and superior alignment with regulatory standards.

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Boosted AAV Vector Purification

Adeno-associated viruses (AAVs) — non-enveloped viral vectors — have a tendency to cluster because of their capsids' sticky nature. High salt levels can diminish these clusters, streamlining the purification process. In these conditions, SAN HQ GMP operates proficiently, ensuring a more successful purification outcome.

Adeno-associated viruses (AAVs) — non-enveloped viral vectors — have a tendency to cluster because of their capsids' sticky nature. High salt levels can diminish these clusters, streamlining the purification process. In these conditions, SAN HQ GMP operates proficiently, ensuring a more successful purification outcome.

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Streamlined Workflow

By implementing a salt-resilient enzyme like SAN HQ GMP, you eliminate the necessity for desalting stages before and after nuclease treatment. This streamlines the bioprocessing workflow, resulting in time savings and potential cost reductions.

By implementing a salt-resilient enzyme like SAN HQ GMP, you eliminate the necessity for desalting stages before and after nuclease treatment. This streamlines the bioprocessing workflow, resulting in time savings and potential cost reductions.

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Potential Surge in Virus Yield

Research indicates that elevated salt conditions may boost the titer yield of AAV production. Operating under these conditions, a salt-resilient nuclease like SAN HQ GMP may contribute to an amplified virus yield.

Research indicates that elevated salt conditions may boost the titer yield of AAV production. Operating under these conditions, a salt-resilient nuclease like SAN HQ GMP may contribute to an amplified virus yield.

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Economized Enzyme Usage

Utilizing a salt-resilient enzyme like SAN HQ GMP can decrease enzyme usage, as it continues to function effectively under high salt levels. This could lead to cost savings and an increased efficiency of the process.

Utilizing a salt-resilient enzyme like SAN HQ GMP can decrease enzyme usage, as it continues to function effectively under high salt levels. This could lead to cost savings and an increased efficiency of the process.

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Minimized Risk of Process Disruptions

With a reduced sensitivity to salt's inhibitory effects, SAN HQ GMP ensures consistent and reliable performance across various bioprocessing conditions. This reduces the risk of process disruptions due to enzyme inhibition.

With a reduced sensitivity to salt's inhibitory effects, SAN HQ GMP ensures consistent and reliable performance across various bioprocessing conditions. This reduces the risk of process disruptions due to enzyme inhibition.

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Advantages of minimized residual DNA in lysate

Reducing residual DNA in your lysate is crucial for both process efficiency and product yield. With SAN HQ GMP, you can achieve lower levels of residual DNA, resulting in multiple benefits for your operation.

Adherence to Regulatory Compliance

Regulatory bodies such as the FDA impose strict limits on residual DNA in gene therapy products. SAN HQ GMP can aid in effectively digesting such DNA, assisting manufacturers in adhering to these regulations and sidestepping potential regulatory challenges.

Regulatory bodies such as the FDA impose strict limits on residual DNA in gene therapy products. SAN HQ GMP can aid in effectively digesting such DNA, assisting manufacturers in adhering to these regulations and sidestepping potential regulatory challenges.

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Enhanced Product Safety

Host cell residual DNA in viral vector production could contain sequences potentially harmful when transferred to patients, including oncogenes which could elevate cancer risks. SAN HQ GMP role in reducing residual DNA can bolster the safety profile of gene therapy products.

Host cell residual DNA in viral vector production could contain sequences potentially harmful when transferred to patients, including oncogenes which could elevate cancer risks. SAN HQ GMP role in reducing residual DNA can bolster the safety profile of gene therapy products. Host cell residual DNA in viral vector production could contain sequences potentially harmful when transferred to patients, including oncogenes which could elevate cancer risks. SAN HQ GMP role in reducing residual DNA can bolster the safety profile of gene therapy products.

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Elevated Product Purity

Lower residual DNA levels enhance the final product's purity. This improvement could enhance the product's effectiveness and mitigate the risk of adverse immune responses in patients.

Lower residual DNA levels enhance the final product's purity. This improvement could enhance the product's effectiveness and mitigate the risk of adverse immune responses in patients.

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Efficiency in Production Process

Efficient residual DNA digestion could streamline the downstream purification process. Large amounts of DNA can create viscosity challenges that complicate filtration and other purification stages.

Efficient residual DNA digestion could streamline the downstream purification process. Large amounts of DNA can create viscosity challenges that complicate filtration and other purification stages.

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Superior Product Quality

By aiding in residual DNA digestion, SAN HQ GMP ensures the final gene therapy product consists primarily of therapeutic viral vectors, leading to a more consistent and superior-quality product.

By aiding in residual DNA digestion, SAN HQ GMP ensures the final gene therapy product consists primarily of therapeutic viral vectors, leading to a more consistent and superior-quality product.

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Boosted Yield

Efficient digestion of residual DNA may enhance the yield of the desired product. DNA binding to viral vectors can diminish the effective yield. By digesting this DNA, SAN HQ GMP can help maximize viral vector yield.

Efficient digestion of residual DNA may enhance the yield of the desired product. DNA binding to viral vectors can diminish the effective yield. By digesting this DNA, SAN HQ GMP can help maximize viral vector yield.

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Sept 2023 DMF Approved by FDA
GMP processes Compliant
Q1 2024
SAN HQ GMP
Ready for Use!
Salt Active
Elisa Kit
GMP Grade
SAN HQ GMP
Benzonase GMP
Denarase GMP