Viscosity Reduction
Application Overview
Viscosity reduction in bacterial lysates is a critical step in various biotechnological processes, including purification of proteins, viral vectors and other biological components produced by host cells. Salt-Active Nucleases offer a targeted approach to reduce viscosity in lysates from host-cells, adaptable to the different salt concentrations often used to maintain solubility.
Application Overview
Viscosity reduction in bacterial lysates is a critical step in various biotechnological processes, including purification of proteins, viral vectors and other biological components produced by host cells. Salt-Active Nucleases offer a targeted approach to reduce viscosity in lysates from host-cells, adaptable to the different salt concentrations often used to maintain solubility.
THE PROBLEM These ENZYMEs SOLVE
High viscosity in cell lysates can impede downstream processes, affecting the efficiency and accuracy of applications like protein purification. Traditional methods for viscosity reduction can be time-consuming and may not be effective in all types.
THE PROBLEM These ENZYME SOLVES
High viscosity in cell lysates can impede downstream processes, affecting the efficiency and accuracy of applications like protein purification. Traditional methods for viscosity reduction can be time-consuming and may not be effective in all types.
THE PROBLEM These ENZYMes SOLVE
High viscosity in cell lysates can impede downstream processes, affecting the efficiency and accuracy of applications like protein purification. Traditional methods for viscosity reduction can be time-consuming and may not be effective in all types.
The Solution
SAN enzymes provide a quick and efficient method for reducing viscosity in bacterial lysates. The amount of SAN required varies depending on the NaCl concentration in the lysate. For lysates containing 0.5 M NaCl, adding 25 U SAN per mL (or 125 U/g wet weight of bacteria) and incubating at room temperature for 10-20 minutes is usually sufficient. In contrast, lysates without NaCl may require up to 10 times more SAN for effective viscosity reduction. For example, 1000 U per mL may be needed for lysates without NaCl, compared to 100 U per mL for lysates with 0.25 M NaCl.
Application Background
