Shrimp Alkaline Phosphatase (SAP)

Shrimp Alkaline Phosphatase (SAP) has become one of today’s most-selling DNA modifying enzymes due to the added convenience through a complete heat inactivation. While other alkaline phosphatases (from E. coli or Calf intestine) must be removed by extraction procedures, SAP is completely inactivated after 15 minutes at 65°C. SAP works well in common buffers without the requirement for other additions.

Main advantages with SAP

• Very high specific activity
• 100% heat-inactivated at 65°C
• Removes 5’-phosphates from DNA, RNA, dNTPs and proteins
• May be added directly to restriction enzyme digests
• No vector purification necessary
• Requires no supplemental zinc or other additives for activity
• Works directly in many different buffers
• Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis