Salt Active Nuclease (SAN)
In protein studies DNA often poses a problem as it creates viscosity in the sample and interferes with downstream protein analysis. To limit protein-DNA interactions, the ionic strength may be raised by the addition of salts. However, even at moderately high salt concentration most nucleases become inactive, making enzymatic removal of DNA problematic. SAN is a unspecific nuclease active at high salt, making it convenient in removal of DNA from cell-lysates and protein preparations.
